universal type i ifn-a Search Results


ifn a d  (PBL Assay)
97
PBL Assay ifn a d
Ifn A D, supplied by PBL Assay, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
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human recombinant universal ifna a d  (R&D Systems)
94
R&D Systems human recombinant universal ifna a d
Human Recombinant Universal Ifna A D, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/universal+type+i+ifn-a/Recombinant+Human+Universal+Type+I+IFN+Protein%2C+CF/pm35186439-106-17-21
Average 94 stars, based on 1 article reviews
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universal type i ifn  (R&D Systems)
94
R&D Systems universal type i ifn
Universal Type I Ifn, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/universal+type+i+ifn-a/Universal+Type+I+IFN+Protein/pm15265950-56-0-23
Average 94 stars, based on 1 article reviews
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universal type i ifn alpha 11100 1  (PBL Assay)
96
PBL Assay universal type i ifn alpha 11100 1
<t>Type</t> <t>I</t> IFN-induced responses evaluated by RNA sequencing in peripheral blood cells of healthy donors. ( A ) PC analysis was performed using the 500 most variable genes. The percentage of variance retained by the two first components is represented. The donor effect was removed (using the function ‘removeBatchEffect’) from the R package limma, before plotting, and the gene expression levels were normalized using the variance-stabilizing transformation. Samples are colored by the time after exposure to IFN-α. Time is represented in hours. ( B ) Heatmap of the top DEGs (log 2 fold change > 1 and adjusted p value < 0.01 compared with baseline) grouped using hierarchical clustering. The gene expression values were scaled using a Z-score normalization (for each condition, the gene expression value was subtracted from the gene average value and divided by its SD [x – mean/SD]). ( C ) Total number of DEGs (log 2 fold change > 1 and adjusted p value < 0.05) induced by IFN-α at each time point. ( D ) Volcano plot of DEGs affected by IFN-α + placebo at 9 h vs. placebo at baseline. Each gene was plotted as log 2 fold change (x-axis) and −log 10 (adjusted p value [FDR]) (y-axis). The horizontal dashed line indicates the cutoff of −log 10 (adjusted p value) of > 1.3 (adjusted p value < 0.05), and the vertical dashed lines indicate log 2 fold change > 1. ( E ) Gene set enrichment analysis following differential gene expression analysis of peripheral blood cells exposed to placebo + IFN-α at 9 h vs. placebo at 0 h. Hallmark gene sets upregulated (red) and downregulated (blue) are shown. Only significantly modified pathways (adjusted p value < 0.05) are shown as bar plots with the normalized enrichment score on the x-axis. Abbreviations: DEG, differentially expressed gene; FDR, false discovery rate; IFN, interferon; IL, interleukin; JAK, Janus kinase; PC, principal component; SD, standard deviation; STAT, signal transducer and activator of transcription; TNF-α, tumor necrosis factor-α
Universal Type I Ifn Alpha 11100 1, supplied by PBL Assay, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/universal+type+i+ifn-a/Human+Interferon+Alpha+A+-+Alpha+2a/pmc13346301-165-16-21
Average 96 stars, based on 1 article reviews
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human universal type i ifn a2  (PBL Assay)
95
PBL Assay human universal type i ifn a2
<t>Type</t> <t>I</t> IFN-induced responses evaluated by RNA sequencing in peripheral blood cells of healthy donors. ( A ) PC analysis was performed using the 500 most variable genes. The percentage of variance retained by the two first components is represented. The donor effect was removed (using the function ‘removeBatchEffect’) from the R package limma, before plotting, and the gene expression levels were normalized using the variance-stabilizing transformation. Samples are colored by the time after exposure to IFN-α. Time is represented in hours. ( B ) Heatmap of the top DEGs (log 2 fold change > 1 and adjusted p value < 0.01 compared with baseline) grouped using hierarchical clustering. The gene expression values were scaled using a Z-score normalization (for each condition, the gene expression value was subtracted from the gene average value and divided by its SD [x – mean/SD]). ( C ) Total number of DEGs (log 2 fold change > 1 and adjusted p value < 0.05) induced by IFN-α at each time point. ( D ) Volcano plot of DEGs affected by IFN-α + placebo at 9 h vs. placebo at baseline. Each gene was plotted as log 2 fold change (x-axis) and −log 10 (adjusted p value [FDR]) (y-axis). The horizontal dashed line indicates the cutoff of −log 10 (adjusted p value) of > 1.3 (adjusted p value < 0.05), and the vertical dashed lines indicate log 2 fold change > 1. ( E ) Gene set enrichment analysis following differential gene expression analysis of peripheral blood cells exposed to placebo + IFN-α at 9 h vs. placebo at 0 h. Hallmark gene sets upregulated (red) and downregulated (blue) are shown. Only significantly modified pathways (adjusted p value < 0.05) are shown as bar plots with the normalized enrichment score on the x-axis. Abbreviations: DEG, differentially expressed gene; FDR, false discovery rate; IFN, interferon; IL, interleukin; JAK, Janus kinase; PC, principal component; SD, standard deviation; STAT, signal transducer and activator of transcription; TNF-α, tumor necrosis factor-α
Human Universal Type I Ifn A2, supplied by PBL Assay, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/universal+type+i+ifn-a/Human+Interferon+Alpha+2/pm35381074-43-60-65
Average 95 stars, based on 1 article reviews
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phanta max super-fidelity dna polymerase  (Vazyme Biotech Co)
97
Vazyme Biotech Co phanta max super-fidelity dna polymerase
<t>Type</t> <t>I</t> IFN-induced responses evaluated by RNA sequencing in peripheral blood cells of healthy donors. ( A ) PC analysis was performed using the 500 most variable genes. The percentage of variance retained by the two first components is represented. The donor effect was removed (using the function ‘removeBatchEffect’) from the R package limma, before plotting, and the gene expression levels were normalized using the variance-stabilizing transformation. Samples are colored by the time after exposure to IFN-α. Time is represented in hours. ( B ) Heatmap of the top DEGs (log 2 fold change > 1 and adjusted p value < 0.01 compared with baseline) grouped using hierarchical clustering. The gene expression values were scaled using a Z-score normalization (for each condition, the gene expression value was subtracted from the gene average value and divided by its SD [x – mean/SD]). ( C ) Total number of DEGs (log 2 fold change > 1 and adjusted p value < 0.05) induced by IFN-α at each time point. ( D ) Volcano plot of DEGs affected by IFN-α + placebo at 9 h vs. placebo at baseline. Each gene was plotted as log 2 fold change (x-axis) and −log 10 (adjusted p value [FDR]) (y-axis). The horizontal dashed line indicates the cutoff of −log 10 (adjusted p value) of > 1.3 (adjusted p value < 0.05), and the vertical dashed lines indicate log 2 fold change > 1. ( E ) Gene set enrichment analysis following differential gene expression analysis of peripheral blood cells exposed to placebo + IFN-α at 9 h vs. placebo at 0 h. Hallmark gene sets upregulated (red) and downregulated (blue) are shown. Only significantly modified pathways (adjusted p value < 0.05) are shown as bar plots with the normalized enrichment score on the x-axis. Abbreviations: DEG, differentially expressed gene; FDR, false discovery rate; IFN, interferon; IL, interleukin; JAK, Janus kinase; PC, principal component; SD, standard deviation; STAT, signal transducer and activator of transcription; TNF-α, tumor necrosis factor-α
Phanta Max Super Fidelity Dna Polymerase, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
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recombinant human ifna-a/d  (PBL Biomedical Laboratories)
90
PBL Biomedical Laboratories recombinant human ifna-a/d
<t>Type</t> <t>I</t> IFN-induced responses evaluated by RNA sequencing in peripheral blood cells of healthy donors. ( A ) PC analysis was performed using the 500 most variable genes. The percentage of variance retained by the two first components is represented. The donor effect was removed (using the function ‘removeBatchEffect’) from the R package limma, before plotting, and the gene expression levels were normalized using the variance-stabilizing transformation. Samples are colored by the time after exposure to IFN-α. Time is represented in hours. ( B ) Heatmap of the top DEGs (log 2 fold change > 1 and adjusted p value < 0.01 compared with baseline) grouped using hierarchical clustering. The gene expression values were scaled using a Z-score normalization (for each condition, the gene expression value was subtracted from the gene average value and divided by its SD [x – mean/SD]). ( C ) Total number of DEGs (log 2 fold change > 1 and adjusted p value < 0.05) induced by IFN-α at each time point. ( D ) Volcano plot of DEGs affected by IFN-α + placebo at 9 h vs. placebo at baseline. Each gene was plotted as log 2 fold change (x-axis) and −log 10 (adjusted p value [FDR]) (y-axis). The horizontal dashed line indicates the cutoff of −log 10 (adjusted p value) of > 1.3 (adjusted p value < 0.05), and the vertical dashed lines indicate log 2 fold change > 1. ( E ) Gene set enrichment analysis following differential gene expression analysis of peripheral blood cells exposed to placebo + IFN-α at 9 h vs. placebo at 0 h. Hallmark gene sets upregulated (red) and downregulated (blue) are shown. Only significantly modified pathways (adjusted p value < 0.05) are shown as bar plots with the normalized enrichment score on the x-axis. Abbreviations: DEG, differentially expressed gene; FDR, false discovery rate; IFN, interferon; IL, interleukin; JAK, Janus kinase; PC, principal component; SD, standard deviation; STAT, signal transducer and activator of transcription; TNF-α, tumor necrosis factor-α
Recombinant Human Ifna A/D, supplied by PBL Biomedical Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/universal+type+i+ifn-a/recombinant+ifn+%CE%B1/pm23505563-41-0-13
Average 90 stars, based on 1 article reviews
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unmasking solution  (Vector Laboratories)
96
Vector Laboratories unmasking solution
<t>Type</t> <t>I</t> IFN-induced responses evaluated by RNA sequencing in peripheral blood cells of healthy donors. ( A ) PC analysis was performed using the 500 most variable genes. The percentage of variance retained by the two first components is represented. The donor effect was removed (using the function ‘removeBatchEffect’) from the R package limma, before plotting, and the gene expression levels were normalized using the variance-stabilizing transformation. Samples are colored by the time after exposure to IFN-α. Time is represented in hours. ( B ) Heatmap of the top DEGs (log 2 fold change > 1 and adjusted p value < 0.01 compared with baseline) grouped using hierarchical clustering. The gene expression values were scaled using a Z-score normalization (for each condition, the gene expression value was subtracted from the gene average value and divided by its SD [x – mean/SD]). ( C ) Total number of DEGs (log 2 fold change > 1 and adjusted p value < 0.05) induced by IFN-α at each time point. ( D ) Volcano plot of DEGs affected by IFN-α + placebo at 9 h vs. placebo at baseline. Each gene was plotted as log 2 fold change (x-axis) and −log 10 (adjusted p value [FDR]) (y-axis). The horizontal dashed line indicates the cutoff of −log 10 (adjusted p value) of > 1.3 (adjusted p value < 0.05), and the vertical dashed lines indicate log 2 fold change > 1. ( E ) Gene set enrichment analysis following differential gene expression analysis of peripheral blood cells exposed to placebo + IFN-α at 9 h vs. placebo at 0 h. Hallmark gene sets upregulated (red) and downregulated (blue) are shown. Only significantly modified pathways (adjusted p value < 0.05) are shown as bar plots with the normalized enrichment score on the x-axis. Abbreviations: DEG, differentially expressed gene; FDR, false discovery rate; IFN, interferon; IL, interleukin; JAK, Janus kinase; PC, principal component; SD, standard deviation; STAT, signal transducer and activator of transcription; TNF-α, tumor necrosis factor-α
Unmasking Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/universal+type+i+ifn-a/Antigen+Unmasking+Solution%2C+Tris-Based/pm17277125-50-53-57
Average 96 stars, based on 1 article reviews
unmasking solution - by Bioz Stars, 2026-11
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vectastain abc kit  (Vector Laboratories)
96
Vector Laboratories vectastain abc kit
<t>Type</t> <t>I</t> IFN-induced responses evaluated by RNA sequencing in peripheral blood cells of healthy donors. ( A ) PC analysis was performed using the 500 most variable genes. The percentage of variance retained by the two first components is represented. The donor effect was removed (using the function ‘removeBatchEffect’) from the R package limma, before plotting, and the gene expression levels were normalized using the variance-stabilizing transformation. Samples are colored by the time after exposure to IFN-α. Time is represented in hours. ( B ) Heatmap of the top DEGs (log 2 fold change > 1 and adjusted p value < 0.01 compared with baseline) grouped using hierarchical clustering. The gene expression values were scaled using a Z-score normalization (for each condition, the gene expression value was subtracted from the gene average value and divided by its SD [x – mean/SD]). ( C ) Total number of DEGs (log 2 fold change > 1 and adjusted p value < 0.05) induced by IFN-α at each time point. ( D ) Volcano plot of DEGs affected by IFN-α + placebo at 9 h vs. placebo at baseline. Each gene was plotted as log 2 fold change (x-axis) and −log 10 (adjusted p value [FDR]) (y-axis). The horizontal dashed line indicates the cutoff of −log 10 (adjusted p value) of > 1.3 (adjusted p value < 0.05), and the vertical dashed lines indicate log 2 fold change > 1. ( E ) Gene set enrichment analysis following differential gene expression analysis of peripheral blood cells exposed to placebo + IFN-α at 9 h vs. placebo at 0 h. Hallmark gene sets upregulated (red) and downregulated (blue) are shown. Only significantly modified pathways (adjusted p value < 0.05) are shown as bar plots with the normalized enrichment score on the x-axis. Abbreviations: DEG, differentially expressed gene; FDR, false discovery rate; IFN, interferon; IL, interleukin; JAK, Janus kinase; PC, principal component; SD, standard deviation; STAT, signal transducer and activator of transcription; TNF-α, tumor necrosis factor-α
Vectastain Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/universal+type+i+ifn-a/VECTASTAIN+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pm17277125-50-45-57
Average 96 stars, based on 1 article reviews
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control rabbit igg  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology control rabbit igg
<t>Type</t> <t>I</t> IFN-induced responses evaluated by RNA sequencing in peripheral blood cells of healthy donors. ( A ) PC analysis was performed using the 500 most variable genes. The percentage of variance retained by the two first components is represented. The donor effect was removed (using the function ‘removeBatchEffect’) from the R package limma, before plotting, and the gene expression levels were normalized using the variance-stabilizing transformation. Samples are colored by the time after exposure to IFN-α. Time is represented in hours. ( B ) Heatmap of the top DEGs (log 2 fold change > 1 and adjusted p value < 0.01 compared with baseline) grouped using hierarchical clustering. The gene expression values were scaled using a Z-score normalization (for each condition, the gene expression value was subtracted from the gene average value and divided by its SD [x – mean/SD]). ( C ) Total number of DEGs (log 2 fold change > 1 and adjusted p value < 0.05) induced by IFN-α at each time point. ( D ) Volcano plot of DEGs affected by IFN-α + placebo at 9 h vs. placebo at baseline. Each gene was plotted as log 2 fold change (x-axis) and −log 10 (adjusted p value [FDR]) (y-axis). The horizontal dashed line indicates the cutoff of −log 10 (adjusted p value) of > 1.3 (adjusted p value < 0.05), and the vertical dashed lines indicate log 2 fold change > 1. ( E ) Gene set enrichment analysis following differential gene expression analysis of peripheral blood cells exposed to placebo + IFN-α at 9 h vs. placebo at 0 h. Hallmark gene sets upregulated (red) and downregulated (blue) are shown. Only significantly modified pathways (adjusted p value < 0.05) are shown as bar plots with the normalized enrichment score on the x-axis. Abbreviations: DEG, differentially expressed gene; FDR, false discovery rate; IFN, interferon; IL, interleukin; JAK, Janus kinase; PC, principal component; SD, standard deviation; STAT, signal transducer and activator of transcription; TNF-α, tumor necrosis factor-α
Control Rabbit Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s1378 universal type  (Selleck Chemicals)
96
Selleck Chemicals s1378 universal type
<t>Type</t> <t>I</t> IFN-induced responses evaluated by RNA sequencing in peripheral blood cells of healthy donors. ( A ) PC analysis was performed using the 500 most variable genes. The percentage of variance retained by the two first components is represented. The donor effect was removed (using the function ‘removeBatchEffect’) from the R package limma, before plotting, and the gene expression levels were normalized using the variance-stabilizing transformation. Samples are colored by the time after exposure to IFN-α. Time is represented in hours. ( B ) Heatmap of the top DEGs (log 2 fold change > 1 and adjusted p value < 0.01 compared with baseline) grouped using hierarchical clustering. The gene expression values were scaled using a Z-score normalization (for each condition, the gene expression value was subtracted from the gene average value and divided by its SD [x – mean/SD]). ( C ) Total number of DEGs (log 2 fold change > 1 and adjusted p value < 0.05) induced by IFN-α at each time point. ( D ) Volcano plot of DEGs affected by IFN-α + placebo at 9 h vs. placebo at baseline. Each gene was plotted as log 2 fold change (x-axis) and −log 10 (adjusted p value [FDR]) (y-axis). The horizontal dashed line indicates the cutoff of −log 10 (adjusted p value) of > 1.3 (adjusted p value < 0.05), and the vertical dashed lines indicate log 2 fold change > 1. ( E ) Gene set enrichment analysis following differential gene expression analysis of peripheral blood cells exposed to placebo + IFN-α at 9 h vs. placebo at 0 h. Hallmark gene sets upregulated (red) and downregulated (blue) are shown. Only significantly modified pathways (adjusted p value < 0.05) are shown as bar plots with the normalized enrichment score on the x-axis. Abbreviations: DEG, differentially expressed gene; FDR, false discovery rate; IFN, interferon; IL, interleukin; JAK, Janus kinase; PC, principal component; SD, standard deviation; STAT, signal transducer and activator of transcription; TNF-α, tumor necrosis factor-α
S1378 Universal Type, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/universal+type+i+ifn-a/Ruxolitinib/pm39102336-224-293-290
Average 96 stars, based on 1 article reviews
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cell lysis buffer cell signaling technology  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc cell lysis buffer cell signaling technology
<t>Type</t> <t>I</t> IFN-induced responses evaluated by RNA sequencing in peripheral blood cells of healthy donors. ( A ) PC analysis was performed using the 500 most variable genes. The percentage of variance retained by the two first components is represented. The donor effect was removed (using the function ‘removeBatchEffect’) from the R package limma, before plotting, and the gene expression levels were normalized using the variance-stabilizing transformation. Samples are colored by the time after exposure to IFN-α. Time is represented in hours. ( B ) Heatmap of the top DEGs (log 2 fold change > 1 and adjusted p value < 0.01 compared with baseline) grouped using hierarchical clustering. The gene expression values were scaled using a Z-score normalization (for each condition, the gene expression value was subtracted from the gene average value and divided by its SD [x – mean/SD]). ( C ) Total number of DEGs (log 2 fold change > 1 and adjusted p value < 0.05) induced by IFN-α at each time point. ( D ) Volcano plot of DEGs affected by IFN-α + placebo at 9 h vs. placebo at baseline. Each gene was plotted as log 2 fold change (x-axis) and −log 10 (adjusted p value [FDR]) (y-axis). The horizontal dashed line indicates the cutoff of −log 10 (adjusted p value) of > 1.3 (adjusted p value < 0.05), and the vertical dashed lines indicate log 2 fold change > 1. ( E ) Gene set enrichment analysis following differential gene expression analysis of peripheral blood cells exposed to placebo + IFN-α at 9 h vs. placebo at 0 h. Hallmark gene sets upregulated (red) and downregulated (blue) are shown. Only significantly modified pathways (adjusted p value < 0.05) are shown as bar plots with the normalized enrichment score on the x-axis. Abbreviations: DEG, differentially expressed gene; FDR, false discovery rate; IFN, interferon; IL, interleukin; JAK, Janus kinase; PC, principal component; SD, standard deviation; STAT, signal transducer and activator of transcription; TNF-α, tumor necrosis factor-α
Cell Lysis Buffer Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Type I IFN-induced responses evaluated by RNA sequencing in peripheral blood cells of healthy donors. ( A ) PC analysis was performed using the 500 most variable genes. The percentage of variance retained by the two first components is represented. The donor effect was removed (using the function ‘removeBatchEffect’) from the R package limma, before plotting, and the gene expression levels were normalized using the variance-stabilizing transformation. Samples are colored by the time after exposure to IFN-α. Time is represented in hours. ( B ) Heatmap of the top DEGs (log 2 fold change > 1 and adjusted p value < 0.01 compared with baseline) grouped using hierarchical clustering. The gene expression values were scaled using a Z-score normalization (for each condition, the gene expression value was subtracted from the gene average value and divided by its SD [x – mean/SD]). ( C ) Total number of DEGs (log 2 fold change > 1 and adjusted p value < 0.05) induced by IFN-α at each time point. ( D ) Volcano plot of DEGs affected by IFN-α + placebo at 9 h vs. placebo at baseline. Each gene was plotted as log 2 fold change (x-axis) and −log 10 (adjusted p value [FDR]) (y-axis). The horizontal dashed line indicates the cutoff of −log 10 (adjusted p value) of > 1.3 (adjusted p value < 0.05), and the vertical dashed lines indicate log 2 fold change > 1. ( E ) Gene set enrichment analysis following differential gene expression analysis of peripheral blood cells exposed to placebo + IFN-α at 9 h vs. placebo at 0 h. Hallmark gene sets upregulated (red) and downregulated (blue) are shown. Only significantly modified pathways (adjusted p value < 0.05) are shown as bar plots with the normalized enrichment score on the x-axis. Abbreviations: DEG, differentially expressed gene; FDR, false discovery rate; IFN, interferon; IL, interleukin; JAK, Janus kinase; PC, principal component; SD, standard deviation; STAT, signal transducer and activator of transcription; TNF-α, tumor necrosis factor-α

Journal: Inflammation

Article Title: Pharmacological Characterization of GLPG3667, a Tyrosine Kinase 2-Selective Inhibitor, for the Treatment of Inflammatory and Autoimmune Diseases

doi: 10.1007/s10753-026-02519-1

Figure Lengend Snippet: Type I IFN-induced responses evaluated by RNA sequencing in peripheral blood cells of healthy donors. ( A ) PC analysis was performed using the 500 most variable genes. The percentage of variance retained by the two first components is represented. The donor effect was removed (using the function ‘removeBatchEffect’) from the R package limma, before plotting, and the gene expression levels were normalized using the variance-stabilizing transformation. Samples are colored by the time after exposure to IFN-α. Time is represented in hours. ( B ) Heatmap of the top DEGs (log 2 fold change > 1 and adjusted p value < 0.01 compared with baseline) grouped using hierarchical clustering. The gene expression values were scaled using a Z-score normalization (for each condition, the gene expression value was subtracted from the gene average value and divided by its SD [x – mean/SD]). ( C ) Total number of DEGs (log 2 fold change > 1 and adjusted p value < 0.05) induced by IFN-α at each time point. ( D ) Volcano plot of DEGs affected by IFN-α + placebo at 9 h vs. placebo at baseline. Each gene was plotted as log 2 fold change (x-axis) and −log 10 (adjusted p value [FDR]) (y-axis). The horizontal dashed line indicates the cutoff of −log 10 (adjusted p value) of > 1.3 (adjusted p value < 0.05), and the vertical dashed lines indicate log 2 fold change > 1. ( E ) Gene set enrichment analysis following differential gene expression analysis of peripheral blood cells exposed to placebo + IFN-α at 9 h vs. placebo at 0 h. Hallmark gene sets upregulated (red) and downregulated (blue) are shown. Only significantly modified pathways (adjusted p value < 0.05) are shown as bar plots with the normalized enrichment score on the x-axis. Abbreviations: DEG, differentially expressed gene; FDR, false discovery rate; IFN, interferon; IL, interleukin; JAK, Janus kinase; PC, principal component; SD, standard deviation; STAT, signal transducer and activator of transcription; TNF-α, tumor necrosis factor-α

Article Snippet: After the incubation period, release of IFN-γ-induced protein 10 (IP-10) was triggered with 3000 U/mL IFN-α (Universal Type I IFN-Alpha, 11100-1, PBL Assay Science, PBL) for 24 h at 37 °C.

Techniques: RNA Sequencing, Gene Expression, Transformation Assay, Modification, Standard Deviation